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full length biotinylated spike protein  (Sino Biological)
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Sino Biological full length biotinylated spike protein
( A ) Dot plots representing expansion of spike + cells within total CD20 + B cells in PBMCs before and after vaccination (aggregate differences at baseline, n = 5, and following vaccination, n = 8, on the left; matched differences on the right, n = 4). PBMCs were incubated with <t>biotinylated</t> spike protein and fluorochrome-conjugated streptavidin, surface stained, washed, and analyzed using flow cytometry. Group differences were tested using unpaired t test with Welch’s correction (left) or paired t test (right). Error bars denote medians and interquartile ranges. ( B ) Magnified image of B cell subsets identified using scRNA-Seq. Data include samples from all 4 groups. ( C ) Pie chart quantifying B cell cluster frequencies after infection and vaccination. ( D ) Isotype distribution of productive B cell clones in vaccinated ( n = 4) and convalescent ( n = 3) individuals. Isotypes were determined based on the constant region of the clone. ( E ) Aggregate clonal abundance following vaccination and infection. ( F ) Volcano plots depicting heavy chain gene usage biases following convalescence (relative to preinfection baseline) or vaccination (relative to prevaccination baseline). The x axis represents the change in gene usage, and the y axis represents P value (–log 10 ). * P < 0.05.
Full Length Biotinylated Spike Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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full length biotinylated spike protein - by Bioz Stars, 2026-03
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( A ) Dot plots representing expansion of spike + cells within total CD20 + B cells in PBMCs before and after vaccination (aggregate differences at baseline, n = 5, and following vaccination, n = 8, on the left; matched differences on the right, n = 4). PBMCs were incubated with biotinylated spike protein and fluorochrome-conjugated streptavidin, surface stained, washed, and analyzed using flow cytometry. Group differences were tested using unpaired t test with Welch’s correction (left) or paired t test (right). Error bars denote medians and interquartile ranges. ( B ) Magnified image of B cell subsets identified using scRNA-Seq. Data include samples from all 4 groups. ( C ) Pie chart quantifying B cell cluster frequencies after infection and vaccination. ( D ) Isotype distribution of productive B cell clones in vaccinated ( n = 4) and convalescent ( n = 3) individuals. Isotypes were determined based on the constant region of the clone. ( E ) Aggregate clonal abundance following vaccination and infection. ( F ) Volcano plots depicting heavy chain gene usage biases following convalescence (relative to preinfection baseline) or vaccination (relative to prevaccination baseline). The x axis represents the change in gene usage, and the y axis represents P value (–log 10 ). * P < 0.05.

Journal: JCI Insight

Article Title: Single-cell profiling of T and B cell repertoires following SARS-CoV-2 mRNA vaccine

doi: 10.1172/jci.insight.153201

Figure Lengend Snippet: ( A ) Dot plots representing expansion of spike + cells within total CD20 + B cells in PBMCs before and after vaccination (aggregate differences at baseline, n = 5, and following vaccination, n = 8, on the left; matched differences on the right, n = 4). PBMCs were incubated with biotinylated spike protein and fluorochrome-conjugated streptavidin, surface stained, washed, and analyzed using flow cytometry. Group differences were tested using unpaired t test with Welch’s correction (left) or paired t test (right). Error bars denote medians and interquartile ranges. ( B ) Magnified image of B cell subsets identified using scRNA-Seq. Data include samples from all 4 groups. ( C ) Pie chart quantifying B cell cluster frequencies after infection and vaccination. ( D ) Isotype distribution of productive B cell clones in vaccinated ( n = 4) and convalescent ( n = 3) individuals. Isotypes were determined based on the constant region of the clone. ( E ) Aggregate clonal abundance following vaccination and infection. ( F ) Volcano plots depicting heavy chain gene usage biases following convalescence (relative to preinfection baseline) or vaccination (relative to prevaccination baseline). The x axis represents the change in gene usage, and the y axis represents P value (–log 10 ). * P < 0.05.

Article Snippet: To detect antigen-specific B cells, approximately 5 × 10 5 PBMCs were stained with 100 ng full-length biotinylated spike protein (Sino Biological) preincubated with Streptavidin-BV510 (Biolegend) at a 2:1 ratio for 1 hour at 4°C to ensure maximum staining quality before surface staining with CD20-FITC (Biolegend, 2H7) for an additional 30 minutes.

Techniques: Incubation, Staining, Flow Cytometry, Infection, Clone Assay